High-resolution native MS analysis.
TheThermo ScientificTM Q ExactiveTM UHMR Hybrid Quadrupole-Orbitrap Mass Spectrometer is Thermo Fisher Scientific's first platform to combine substantially increased sensitivity and mass resolution at high m/z, with MS2 and pseudo-MS3 capabilities – creating a unique and powerful analytical solution for the investigation of structure and heterogeneity of protein complexes.
With its superior ability to perform native intact mass and top-down analyses, Q Exactive UHMR MS provides unprecedented structural detail which you cannot see with other methods enabling deeper understanding of protein function, disease mechanisms, potential drug targets, and biotherapeutic compounds.
Unique Features & Benefits of the Q Exactive UHMR Hybrid MS:
Confidently resolve small mass differences
Analyze intact MegaDalton assemblies and resolve small differences in masses that reveal key ligands, modifications and interactions for better understanding of biological processes.
Conserve precious sample
With orders of magnitude enhanced sensitivity at high m/z, make high-confidence measurements, even if you have very small amounts of sample to work with.
Perform native top-down analysis of protein complexes
Ultra-high mass quadrupole selection up to 25k m/z and higher fragmentation efficiency in the injection flatapole and HCD cell region allow native top-down analysis.
Characterize intact native protein assemblies, including membrane proteins
By varying the in-source trapping energy, the instrument can release protein subunits for top-down sequencing or, with gentle activation, retain membrane proteins bound to multiple ligands allowing whole complex analysis.
Performance Characteristics of the Q Exactive UHMR Hybrid MS:
Resolution:
200,000 @ m/z 400
Mass range:
m/z 350 to 80,000
Quad mass selection:
Up to m/z 25,000
Scan rate:
12 Hz at resolution setting of 12,500 @ m/z 400
Mass accuracy:
Internal: <1 ppm RMS
External: <3 ppm RMS
Dynamic range:
>5000:1
Analog inputs:
One (1) analog input (0–1 V)
One (1) analog (0–10 V)
יצרן: Thermo Scientific
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