Orbitrap Fusion – Tribrid Mass Spectrometer

Unique Features of the Orbitrap Fusion:

Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion Tribrid mass spectrometer delivers unmatched analytical performance with revolutionary depth of analysis, unprecedented usability, and exceptional robustness.

• Resolution up to 450,000 to remove spectral interferences
• CID/HCD/ETD detected by the Ion Trap or Orbitrap at any level of MSn for maximum experimental flexibility

• Dynamic Scan Management architecture lets every user easily get the maximum information from every sample

Performance Characteristics of the Orbitrap Fusion:

Mass Range:

Linear Ion Trap and Orbitrap Mass Range:

Standard – m/z 50–2,000
High Mass – m/z 200–4,000

Orbitrap Extended Mass Range:

Up to m/z 6000 without precursor ion selection

Resolution:

15,000-450,000 (FWHM) at m/z 200

Scan Rate:

Orbitrap MSn up to 20 Hz
Ion trap MSn up to 20 Hz

Mass Accuracy:

< 3 ppm RMS using external calibration
< 1 ppm RMS using internal calibration

MS/MS Sensitivity of Ion Trap:

2 μL of a 50 fg/μL solution of reserpine (100 fg total) injected at a flow of 500 μL/min will produce a minimum signal-to-noise ratio of 100:1 for the transition of the isolated protonated molecular ions at m/z 609 to the largest two product ions, m/z 397 and m/z 448, when the mass spectrometer is operated at unit resolution in the full-scan MS/MS mode, m/z 165–615. The test requires the HESI probe.

Dynamic Range:

> 5,000 within a single scan, guaranteeing specified mass accuracy

MS Scan Power:

MSn, for n = 1 through 10

Thermo Scientific™ EASY-ETD™ Ion Source:

• Generates fluoranthene anions for electron transfer dissociation (ETD)

  • Townsend discharge ionization is extremely stable and robust

  • Compact size, located entirely within the footprint of the instrument

  • Active reagent ion filtering using mass-resolving quadrupole

Thermo Scientific™ EASY-IC™ Ion Source:

• Generates internal calibrant ions for real-time mass calibration on every spectrum

  • Provides <1 ppm RMS mass accuracy

Synchronous Precursor Selection

Up to 15 precursors per MS2 scan, for MS3 analysis only

Multiplexing Using Ion-Routing Multipole:

Up to 10 precursors per scan using quadrupole mass filter

Polarity Switching:

One full cycle in 1.1 sec (one full scan in positive mode and one full scan in negative mode at resolution setting of 35,000)

יצרן: Thermo Scientific